| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
May, Sophie N (17)
|
MAUN-4 |
24.19 |
26.24 |
2.05 |
| 2 |
Duncan, Marlies A (17)
|
MACAA |
25.40 |
26.69 |
1.29 |
| 3 |
Grubb, Rachel E (15)
|
MACAA |
27.59 |
27.20 |
-0.39 |
| 4 |
Ceribelli, Mia F (15)
|
MACAA |
1:00.00 |
27.79 |
-32.21 |
| 5 |
Eason, Olivia P (15)
|
MAUN-2 |
28.97 |
28.56 |
-0.41 |
| 6 |
Franciscus, Samantha L (18)
|
MAUN-2 |
26.11 |
28.62 |
2.51 |
| 7 |
Franciscus, Kimberly A (15)
|
MAUN-2 |
26.45 |
29.31 |
2.86 |
| 8 |
Koloseike, Claire E (15)
|
MALCY |
27.35 |
29.38 |
2.03 |
| 9 |
Burke, Alexandra R (15)
|
MAUN-3 |
30.26 |
33.39 |
3.13 |
|
Sears, Katherine L (18)
|
MACAA |
25.70 |
NS |
- |
|
Mullins, Kirsten A (17)
|
MAGRA |
28.72 |
NS |
- |
|
Russell, Grace C (17)
|
MAKAY |
27.96 |
NS |
- |
|
Cleveland, Riley C (16)
|
MALCY |
28.90 |
NS |
- |
|
Collier, Tara L (18)
|
MALCY |
25.99 |
NS |
- |
|
McDermott, Breanne (17)
|
MALCY |
26.04 |
NS |
- |